Development and use of methodology for qualification of cancer cell

This paper is on development and use of methodology for qualification of cancer cell and non-cancer cell microscopy images for quantifying cell confluency and phenotype

Development and use of methodology for qualification of cancer cell

Development and use of methodology for qualification of cancer cell and non-cancer cell microscopy images for quantifying cell confluency and phenotype

8000 words final year project for molecular and cellular biology course, 3000 words for introduction.

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Introduction: Why the study of cancer cell migration is of interest and which tools can be useful
for this study Cancer is a major health problem for humankind, and the approaches to its
treatment have clear limitations.

Oncology has one of the poorest records
for investigational drugs in clinical development, with success rates that are more than three times lower than for cardiovascular diseases. Oncology, as a therapeutic area, is thus characterized by a desperate medical need for new drugs of novel types, i.e., not only drugs that kill cells and are consequently
often toxic. The poor performance of most investigational drugs implies that the standard preclinical disease models are faulty or, at least, improperly used.

As emphasized by Gibbs, the past 20 years have seen a
tremendous increase in our knowledge of the molecular mechanisms and
pathophysiology of human cancer. Many of these mechanisms have been exploited as new targets for drug development in the hope that they will display an enhanced level of antitumor activity and will be less toxic as far as the patients are concerned. Unfortunately, very few of these fundamental research-based hopes lead to clinical success. This failure could be (at least partly)

due to the fact that a large majority of compounds that are entering clinical trials for cancer treatment have been selected in vitro on the basis of their cytotoxicity profile. However, cancer kills patients essentially because of the migratory nature of its cells. Indeed, it is now well established that cell migration plays pivotal roles in cancer cell scattering, tissue invasion and metastasis. For example, processes which are essentially responsible for the dismal prognoses of a majority of cancer patients.

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